TRYPANOSOMA CRUZI IN BRAZILIAN WILDLIFE TRAFFICKING RESERVOIR COMPETENCE BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) ASSAY
TÁLIA MISSEN TREMORI ^2,1, NOEME SOUSA ROCHA^2,1, PEDRO FERNÁNDEZ SOTO^2,1, SELENE DANIELA BABBONI^2,1, ANTONIO MURO ALVARÉZ^2,1, JULIO LÓPEZ ABÁN^2,1
1. CIETUS - USAL - Centro de Investigación de Enfermidades Tropicales, Universidad de Salamanca, 2. FMVZ - UNESP - BOTUCATU - Universidade Estadual Paulista "Júlio de Mesquita Filho" - Faculdade de Medicina Veterinária e Zootecnia - Campus de Botucatu, 3. UNIP - Universidade Paulista - Campus Dutra
talia_missen@hotmail.com

Trypanosoma cruzi , an important protozoan parasite for humans and animals, causes Chagas disease, a Neglected Tropical Disease (NTD) that could affect roughly 6-7 million people in the world, mainly on underdeveloped countries. The vector is a blood-sucking insect and so many mammals could be reservoirs. Animal trafficking, smuggling and illegal trade is the fourth most common illegal activity in the world. An important point concerning illegal animal trade and the increasing globalization is that represents a possible vehicle for illness spreading, including zoonosis, creating a health public issue. Hence the diagnosis in endemic regions and limited resources is very important, an alternative is a molecular technique named loop-mediated isothermal amplification (LAMP), and this assay is a one-step amplification reaction that amplifies a target DNA with high specificity, efficiency and rapid under isothermal conditions. The aim of this study is verify the incidence of the zoonotic agent T. cruzi in 50 mammals from animal trafficking using muscle, blood and skin samples, the molecular diagnosis of T. cruzi ware made using the LAMP assay. In our work 50% of animals were positive in LAMP assay to T. cruzi . This analysis could be important to identify reservoirs and the risk about animal trafficking to human health and the use of LAMP assay in fast and trial diagnosis.